Pathway-focused LC-MS/MS metabolomics for research, preclinical, industry, toxicology, nutrition, exposure, and biomarker studies.
Gaikwad Steroidomics Laboratory provides targeted LC-MS/MS metabolomics services for quantitative analysis of biologically important small molecules, pathway biomarkers, exposure markers, conjugates, adducts, and specialized metabolites.
Unlike untargeted metabolomics workflows that generate broad feature lists requiring extensive downstream identification, GSL’s targeted metabolomics platform focuses on known, biologically interpretable analytes measured using LC-MS/MS-based methods. This approach supports research-ready data, pathway-focused interpretation, and custom assay development for academic, biotechnology, pharmaceutical, nutrition, toxicology, and product-development studies.
GSL’s platform covers 450+ targeted LC-MS/MS analytes, including steroidomics, bileomics, one-carbon metabolism, neurotransmitter metabolism, kidney-gut biomarkers, food and dietary biomarkers, environmental exposure markers, and specialized adduct/conjugate biomarkers.
Platform Scope
GSL’s targeted metabolomics platform supports analysis of pathway-specific biomarker groups, including:
- One-carbon, folate, methylation, and transsulfuration metabolites
- Neurotransmitters, neurotransmitter metabolites, adducts, and conjugates
- Kidney, gut, uremic, and cardiometabolic biomarkers
- Biogenic amines and polyamine metabolism
- Food, dietary, botanical, and archaeological residue biomarkers
- Environmental exposure and pesticide-related biomarkers
- Carcinogen exposure markers and selected DNA adduct biomarkers
- Specialized small-molecule adducts, conjugates, and custom analytes
- Steroidomics, bileomics, oxysterols, neurosteroids, and related endocrine metabolites
Why Targeted Metabolomics?
Targeted metabolomics is especially useful when the research question involves specific pathways, known biomarkers, quantitative comparisons, or publication-ready measurement of defined analytes.
GSL’s targeted LC-MS/MS approach is designed to provide:
- Quantitative or semi-quantitative analyte measurements
- Pathway-focused biomarker panels
- Matrix-normalized values when applicable
- Research-ready data tables
- QC-reviewed chromatographic data
- Custom assay development for specialized metabolites
- Optional scientific interpretation and pathway-level discussion
This approach is well suited for studies where biological interpretation, reproducibility, and targeted quantification are more important than broad untargeted feature discovery.
Sample Types
Depending on the project and analyte group, GSL can support targeted metabolomics analysis in:
- Urine
- Serum or plasma
- Saliva
- Tissue
- Cells and cell-culture media
- Feces
- Food, plant, botanical, and archaeological extracts
- Environmental or exposure-related matrices
- Custom biological or research matrices
Sample requirements depend on the matrix, analyte class, concentration range, extraction method, and project scope.
Metabolomics Panels
One-Carbon, Folate & Methylation
Targeted LC-MS/MS analysis of metabolites involved in one-carbon metabolism, folate biology, methylation balance, transsulfuration, and related biochemical pathways.
Applications: methylation research, nutritional studies, metabolic stress, pathway biology, preclinical models, and biomarker discovery.
Neurotransmitter Metabolism, Adducts & Conjugates
Targeted analysis of neurotransmitters, neurotransmitter metabolites, and selected specialized adduct/conjugate biomarkers.
Applications: brain-gut axis research, stress biology, neurochemical metabolism, toxicology, pharmacology, and custom biomarker studies.
Kidney, Gut & Cardiometabolic Biomarkers
Targeted LC-MS/MS analysis of selected kidney-related, gut-derived, uremic, and cardiometabolic biomarkers.
Applications: kidney research, gut-microbiome metabolism, cardiometabolic studies, toxicology, nutrition, and translational biomarker research.
Biogenic Amines & Polyamine Metabolism
Targeted analysis of biogenic amines, polyamines, and related metabolites involved in cellular metabolism, growth biology, microbial activity, and inflammatory or stress-related pathways.
Applications: metabolism research, gut biology, cancer-related research, inflammation, nutrition, and preclinical studies.
Food, Dietary & Archaeological Residue Biomarkers
Targeted LC-MS/MS analysis of selected food-derived, dietary, botanical, and residue biomarkers for nutrition, product-development, exposure, and archaeological research.
Applications: food biomarker studies, dietary exposure, botanical compounds, cacao/chocolate research, residue analysis, and historical or archaeological sample investigations.
Environmental Exposure & Pesticide Biomarkers
Targeted LC-MS/MS analysis of selected environmental exposure markers, pesticide-related biomarkers, and small-molecule exposure indicators.
Applications: toxicology, environmental exposure research, agricultural studies, product safety research, and biomarker monitoring.
Carcinogen Exposure & DNA Adduct Biomarkers
Targeted analysis of selected carcinogen-related biomarkers, reactive metabolite products, and DNA adduct biomarkers.
Applications: chemical carcinogenesis, toxicology, exposure biology, mechanistic research, oxidative stress, and pathway-specific biomarker studies.
Custom Targeted Metabolomics Assay Development
GSL also supports custom LC-MS/MS assay development for specialized research projects. Custom work may include:
- New analyte method development
- Matrix-specific extraction optimization
- Targeted MRM method development
- Standard preparation and calibration strategy
- Adduct or conjugate biomarker analysis
- Small-molecule pathway panel expansion
- Research-ready data tables and reporting formats
Specialized adducts, conjugates, proprietary ratios, and full technical assay details are not fully disclosed publicly but may be available for qualified research clients upon request.
Research Applications
GSL’s targeted metabolomics platform can support:
- Academic research studies
- Preclinical and animal-model studies
- Pharmaceutical and biotechnology research
- Nutrition and dietary biomarker studies
- Food, botanical, and product-development studies
- Toxicology and environmental exposure research
- Kidney, gut, liver, endocrine, and metabolic pathway studies
- Custom biomarker discovery and validation projects
- Publication-focused metabolomics studies
Reporting Options
Depending on the project, GSL reports may include:
- Measured analyte concentrations
- Matrix-normalized values
- Creatinine-normalized urine results when applicable
- Pathway-focused biomarker tables
- Analyte ratios or pathway indices when appropriate
- QC-reviewed chromatographic data
- Publication-ready Excel tables
- Optional scientific interpretation
Important Note
Analyte availability may depend on sample type, sample amount, matrix complexity, expected concentration range, extraction method, standards availability, and project scope. GSL’s targeted metabolomics services are intended for research, preclinical, industrial, product-development, and scientific applications. They are not intended to diagnose, treat, or replace medical care.
Request Targeted Metabolomics Analysis
GSL can help design a targeted metabolomics panel based on your research question, sample type, analyte class, and reporting needs.