Gaikwad Steroidomics Laboratory provides targeted LC-MS/MS bile acid and bileomics analysis for liver, gut, microbiome, kidney, toxicology, endocrine, and preclinical research. Bile acids are steroid-derived metabolites that connect cholesterol metabolism, liver function, gut microbiome activity, enterohepatic circulation, metabolic disease, and tissue-specific internal exposure.
GSL’s bileomics capabilities build on Dr. Gaikwad’s work introducing the concepts of “Bileome” and “Bileomics” for targeted bile acid metabolome analysis. This pathway-focused approach supports quantitative profiling of bile acids and bile-acid-related metabolites across biological matrices and research models.
What This Panel Measures
The Bile Acid & Bileomics Panel supports targeted LC-MS/MS analysis of:
- Primary bile acids
- Secondary bile acids
- Glycine-conjugated bile acids
- Taurine-conjugated bile acids
- Muricholic acids for rodent studies
- Sulfated bile acids
- Hydroxylated and oxidized bile-acid-related metabolites
- Selected bile-acid pathway biomarkers
Representative Analytes
Representative analytes may include:
Cholic acid, chenodeoxycholic acid, deoxycholic acid, lithocholic acid, ursodeoxycholic acid, hyodeoxycholic acid, allolithocholic acid, muricholic acid, α-muricholic acid, β-muricholic acid, taurocholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid, tauroursodeoxycholic acid, taurolithocholic acid, taurohyodeoxycholic acid, tauro-β-muricholic acid, glycocholic acid, glycochenodeoxycholic acid, glycodeoxycholic acid, glycolithocholic acid, glycohyodeoxycholic acid, ursocholic acid, isolithocholic acid, cholic acid sulfate, lithocholic acid sulfate, taurocholic acid sulfate, taurolithocholic acid sulfate, and selected sulfated bile acids.
Additional bile-acid-related analytes may be available depending on sample type, matrix, and project scope.
Sample Types
Representative sample types may include:
- Serum or plasma
- Urine
- Feces
- Bile-related samples
- Liver and intestinal tissues
- Kidney and other tissues
- Cells and culture media
- Preclinical animal model samples
- Biological extracts and custom research matrices
Sample requirements depend on the matrix, expected concentration range, analyte class, and project requirements.
Research Applications
The Bile Acid & Bileomics Panel may support research in:
- Liver disease and cholestasis
- Gut-liver axis biology
- Microbiome and enterohepatic circulation studies
- Kidney and gut-kidney axis research
- Metabolic disease, obesity, and diabetes models
- Drug-induced liver injury and toxicology
- Endocrine and steroid-derived metabolite research
- Preclinical animal studies
- Tissue distribution and internal dose studies
- Custom bile-acid pathway biomarker projects
Bileomics Reporting
Depending on the project, GSL reports may include:
- Individual bile acid concentrations
- Matrix-normalized values when applicable
- Primary and secondary bile acid patterns
- Conjugated and unconjugated bile acid profiles
- Glycine and taurine conjugation patterns
- Rodent-specific muricholic acid profiles
- Sulfated bile acid profiles when applicable
- QC-reviewed data tables
- Publication-ready results
- Optional pathway-focused interpretation
Scientific Foundation
Dr. Gaikwad introduced the concepts of “Bileome” and “Bileomics” in his work on targeted bile acid metabolome analysis.
Selected publications:
- Gaikwad. Bileome: The Bile Acid Metabolome of Rat. Biochemical and Biophysical Research Communications. 533, 458–466, 2020.
- Gaikwad. Bileomic identification and quantification of bile acids in rat kidney by UPLC–MS/MS. Steroids. 2026;230:109777. doi:10.1016/j.steroids.2026.109777.
Important Note
Analyte availability may depend on sample type, sample amount, matrix, concentration range, extraction method, and project requirements. Specialized bile acid metabolites, sulfated bile acids, custom bileomics panels, and matrix-specific assay configurations may be available for qualified research projects upon request.
Request Bileomics Analysis
Contact GSL to discuss bile acid analyte availability, sample requirements, turnaround time, and project pricing.